p p53 ser15 Search Results


p53 [p ser15] antibody - bsa free  (Bio-Techne corporation)
93
Bio-Techne corporation p53 [p ser15] antibody - bsa free
P53 [P Ser15] Antibody Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+p53+ser15/p53+%5Bp+Ser15%5D+Antibody+-+BSA+Free/bio-techne+corporation___nb100-1913
Average 93 stars, based on 1 article reviews
p53 [p ser15] antibody - bsa free - by Bioz Stars, 2026-10
93/100 stars
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phospho p53 ser15  (Novus Biologicals)
85
Novus Biologicals phospho p53 ser15
Figure 1 Different outcomes of the stress response in NIH3T3 cells. (a) FACS analysis: differently irradiated NIH3T3 cells were harvested at the indicated time points after irradiation and stained with propidium iodide to monitor DNA content. (b) Western blot: lysates derived from irradiated NIH3T3 cells at the indicated time points after irradiation were used to monitor the presence of cleaved caspase 3 and its substrate cdc6 as markers of apoptosis. Equal protein loading was confirmed by tubulin expression. (c) X-Gal staining: activity of senescence-associated b-galactosidase was monitored in untreated or 20 Gy gamma-irradiated NIH3T3 cells, harvested 12 days after irradiation. (d) Western blot: lysates from untreated () or irradiated ( þ ) NIH3T3 cells, harvested 8 h after irradiation, were probed for <t>p53</t> and serine 18 phosphorylated p53. Equal loading is shown by a non-specific band of the anti p53- serine18-P antibody (control).
Phospho P53 Ser15, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+p53+ser15/p53+%5Bp+Ser15%5D+Antibody+-+BSA+Free/pm16247471-253-19-31
Average 85 stars, based on 1 article reviews
phospho p53 ser15 - by Bioz Stars, 2026-10
85/100 stars
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anti phospho p53 ser 15  (Novus Biologicals)
86
Novus Biologicals anti phospho p53 ser 15
Figure 1 Different outcomes of the stress response in NIH3T3 cells. (a) FACS analysis: differently irradiated NIH3T3 cells were harvested at the indicated time points after irradiation and stained with propidium iodide to monitor DNA content. (b) Western blot: lysates derived from irradiated NIH3T3 cells at the indicated time points after irradiation were used to monitor the presence of cleaved caspase 3 and its substrate cdc6 as markers of apoptosis. Equal protein loading was confirmed by tubulin expression. (c) X-Gal staining: activity of senescence-associated b-galactosidase was monitored in untreated or 20 Gy gamma-irradiated NIH3T3 cells, harvested 12 days after irradiation. (d) Western blot: lysates from untreated () or irradiated ( þ ) NIH3T3 cells, harvested 8 h after irradiation, were probed for <t>p53</t> and serine 18 phosphorylated p53. Equal loading is shown by a non-specific band of the anti p53- serine18-P antibody (control).
Anti Phospho P53 Ser 15, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+p53+ser15/p53+%5Bp+Ser15%5D+Antibody+(261366)+%5BAllophycocyanin%5D/pm12941605-56-0-4
Average 86 stars, based on 1 article reviews
anti phospho p53 ser 15 - by Bioz Stars, 2026-10
86/100 stars
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p53 [p ser15] antibody (261366) [dylight 594]  (Novus Biologicals)
N/A
The p53 [p Ser15] Antibody (261366) [DyLight 594] from Novus is a p53 antibody to p53. This antibody reacts with Human. The p53 antibody has been validated for the following applications: Intracellular Staining by Flow
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p53 [p ser15] antibody (261366) [dylight 680]  (Novus Biologicals)
N/A
The p53 [p Ser15] Antibody (261366) [DyLight 680] from Novus is a p53 antibody to p53. This antibody reacts with Human. The p53 antibody has been validated for the following applications: Intracellular Staining by Flow
   Buy from Supplier
p53 [p ser15] antibody (261366) [alexa fluor® 594]  (Novus Biologicals)
N/A
The p53 [p Ser15] Antibody (261366) [Alexa Fluor® 594] from Novus is a p53 antibody to p53. This antibody reacts with Human. The p53 antibody has been validated for the following applications: Intracellular Staining by
   Buy from Supplier
p53 [p ser15] antibody (261366) [pe]  (Novus Biologicals)
N/A
The p53 [p Ser15] Antibody (261366) [PE] from Novus is a p53 antibody to p53. This antibody reacts with Human. The p53 antibody has been validated for the following applications: Intracellular Staining by Flow Cytometry,
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p53 [p ser15] antibody (261366) [mfluor violet 500 se]  (Novus Biologicals)
N/A
The p53 [p Ser15] Antibody (261366) [mFluor Violet 500 SE] from Novus is a p53 antibody to p53. This antibody reacts with Human. The p53 antibody has been validated for the following applications: Intracellular Staining
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p53 [p ser15] antibody (261366) [alexa fluor® 350]  (Novus Biologicals)
N/A
The p53 [p Ser15] Antibody (261366) [Alexa Fluor® 350] from Novus is a p53 antibody to p53. This antibody reacts with Human. The p53 antibody has been validated for the following applications: Intracellular Staining by
   Buy from Supplier
p53 [p ser15] antibody (261366) [alexa fluor® 532]  (Novus Biologicals)
N/A
The p53 [p Ser15] Antibody (261366) [Alexa Fluor® 532] from Novus is a p53 antibody to p53. This antibody reacts with Human. The p53 antibody has been validated for the following applications: Intracellular Staining by
   Buy from Supplier
p53 [p ser15] antibody (261366) [dylight 550]  (Novus Biologicals)
N/A
The p53 [p Ser15] Antibody (261366) [DyLight 550] from Novus is a p53 antibody to p53. This antibody reacts with Human. The p53 antibody has been validated for the following applications: Intracellular Staining by Flow
   Buy from Supplier
p53 [p ser15] antibody (261366) [percp]  (Novus Biologicals)
N/A
The p53 [p Ser15] Antibody (261366) [PerCP] from Novus is a p53 antibody to p53. This antibody reacts with Human. The p53 antibody has been validated for the following applications: Intracellular Staining by Flow Cytometry,
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Image Search Results


Figure 1 Different outcomes of the stress response in NIH3T3 cells. (a) FACS analysis: differently irradiated NIH3T3 cells were harvested at the indicated time points after irradiation and stained with propidium iodide to monitor DNA content. (b) Western blot: lysates derived from irradiated NIH3T3 cells at the indicated time points after irradiation were used to monitor the presence of cleaved caspase 3 and its substrate cdc6 as markers of apoptosis. Equal protein loading was confirmed by tubulin expression. (c) X-Gal staining: activity of senescence-associated b-galactosidase was monitored in untreated or 20 Gy gamma-irradiated NIH3T3 cells, harvested 12 days after irradiation. (d) Western blot: lysates from untreated () or irradiated ( þ ) NIH3T3 cells, harvested 8 h after irradiation, were probed for p53 and serine 18 phosphorylated p53. Equal loading is shown by a non-specific band of the anti p53- serine18-P antibody (control).

Journal: Oncogene

Article Title: Dissection of transcriptional and non-transcriptional p53 activities in the response to genotoxic stress.

doi: 10.1038/sj.onc.1209126

Figure Lengend Snippet: Figure 1 Different outcomes of the stress response in NIH3T3 cells. (a) FACS analysis: differently irradiated NIH3T3 cells were harvested at the indicated time points after irradiation and stained with propidium iodide to monitor DNA content. (b) Western blot: lysates derived from irradiated NIH3T3 cells at the indicated time points after irradiation were used to monitor the presence of cleaved caspase 3 and its substrate cdc6 as markers of apoptosis. Equal protein loading was confirmed by tubulin expression. (c) X-Gal staining: activity of senescence-associated b-galactosidase was monitored in untreated or 20 Gy gamma-irradiated NIH3T3 cells, harvested 12 days after irradiation. (d) Western blot: lysates from untreated () or irradiated ( þ ) NIH3T3 cells, harvested 8 h after irradiation, were probed for p53 and serine 18 phosphorylated p53. Equal loading is shown by a non-specific band of the anti p53- serine18-P antibody (control).

Article Snippet: Antibodies were obtained from Upstate: Bax (06- 499); Biomol: Cytochrome c (SA-226); Cell Signaling: Cleaved caspase 3 (9661) and Phospho-p53-Ser15 (9284); BD Pharmingen: p21 (556430); Oncogene: PIG3 (PC268) and Tubulin (CP06); Imgenex: Puma (IMG-459); Santa Cruz: 14-3-3s (sc7681).

Techniques: Irradiation, Staining, Western Blot, Derivative Assay, Expressing, Activity Assay, Control

Figure 2 Influence of p53 and p21 in the response to 20 Gy gamma-irradiation. (a) Western blot: lysates derived from NIH3T3 (wtp53), 10-1 (p53/) and Balb/c3T3tx (mutp53) cells were analysed for p21 expression at the indicated time points after 20 Gy gamma-irradiation. C indicates a control lysate from NIH3T3 cells. Tubulin expression confirmed equal protein loading. (b, d) Western blot: the presence of cleaved caspase 3 was monitored in lysates derived from cells that were harvested 24 h after mock-treatment () or 20 Gy gamma-irradiation ( þ ). MEFs with different genotype (b) and cell lines 10-1 and Balb/c3T3tx (d) were analysed. (c, e) FACS analysis: DNA content was monitored in untreated or 20 Gy gamma-treated MEFs of the indicated genotype (c) and cell lines 10-1 and Balb/c3T3tx (e). Cells were harvested 24 h after irradiation.

Journal: Oncogene

Article Title: Dissection of transcriptional and non-transcriptional p53 activities in the response to genotoxic stress.

doi: 10.1038/sj.onc.1209126

Figure Lengend Snippet: Figure 2 Influence of p53 and p21 in the response to 20 Gy gamma-irradiation. (a) Western blot: lysates derived from NIH3T3 (wtp53), 10-1 (p53/) and Balb/c3T3tx (mutp53) cells were analysed for p21 expression at the indicated time points after 20 Gy gamma-irradiation. C indicates a control lysate from NIH3T3 cells. Tubulin expression confirmed equal protein loading. (b, d) Western blot: the presence of cleaved caspase 3 was monitored in lysates derived from cells that were harvested 24 h after mock-treatment () or 20 Gy gamma-irradiation ( þ ). MEFs with different genotype (b) and cell lines 10-1 and Balb/c3T3tx (d) were analysed. (c, e) FACS analysis: DNA content was monitored in untreated or 20 Gy gamma-treated MEFs of the indicated genotype (c) and cell lines 10-1 and Balb/c3T3tx (e). Cells were harvested 24 h after irradiation.

Article Snippet: Antibodies were obtained from Upstate: Bax (06- 499); Biomol: Cytochrome c (SA-226); Cell Signaling: Cleaved caspase 3 (9661) and Phospho-p53-Ser15 (9284); BD Pharmingen: p21 (556430); Oncogene: PIG3 (PC268) and Tubulin (CP06); Imgenex: Puma (IMG-459); Santa Cruz: 14-3-3s (sc7681).

Techniques: Irradiation, Western Blot, Derivative Assay, Expressing, Control

Figure 3 Expression level of p53 and p53 targets in irradiated NIH3T3 cells. (a-c) Western blot: expression level of anti- and pro- apoptotic p53 targets was analysed in lysates from NIH3T3 cells at the indicated time points after irradiation. Equal protein loading was confirmed by tubulin expression. (b) Puma was detected in lysates of non-irradiated () or irradiated ( þ ) NIH3T3 cells 8 h after irradiation. Equal protein loading is shown by a non-specific band of the anti-p53-ser18-P antibody (control). (c) Lysates of differently irradiated NIH3T3 cells were probed for total p53, serine 18 phosphorylated p53 and Mdm2 at the indicated time points after irradiation.

Journal: Oncogene

Article Title: Dissection of transcriptional and non-transcriptional p53 activities in the response to genotoxic stress.

doi: 10.1038/sj.onc.1209126

Figure Lengend Snippet: Figure 3 Expression level of p53 and p53 targets in irradiated NIH3T3 cells. (a-c) Western blot: expression level of anti- and pro- apoptotic p53 targets was analysed in lysates from NIH3T3 cells at the indicated time points after irradiation. Equal protein loading was confirmed by tubulin expression. (b) Puma was detected in lysates of non-irradiated () or irradiated ( þ ) NIH3T3 cells 8 h after irradiation. Equal protein loading is shown by a non-specific band of the anti-p53-ser18-P antibody (control). (c) Lysates of differently irradiated NIH3T3 cells were probed for total p53, serine 18 phosphorylated p53 and Mdm2 at the indicated time points after irradiation.

Article Snippet: Antibodies were obtained from Upstate: Bax (06- 499); Biomol: Cytochrome c (SA-226); Cell Signaling: Cleaved caspase 3 (9661) and Phospho-p53-Ser15 (9284); BD Pharmingen: p21 (556430); Oncogene: PIG3 (PC268) and Tubulin (CP06); Imgenex: Puma (IMG-459); Santa Cruz: 14-3-3s (sc7681).

Techniques: Expressing, Irradiation, Western Blot, Control

Figure 4 High p53 amounts provide an apoptotic signal. (a) Western blot: 18 h after transfection with GFP or HA-wtp53, NIH3T3 cells were mock () or 20 Gy gamma ( þ ) irradiated and harvested 26 h after irradiation. Lysates were probed for cleaved caspase 3, p53 and p21. Equal protein loading was confirmed by analysis of tubulin expression. (b) Immunostaining: 18 h after transfection with HA-wtp53 or -Gal-GFP, NIH3T3 cells were 20 Gy gamma-irradiated and fixed 26 h after irradiation. Transfected cells were identified with an HA-specific antibody or by GFP-fluorescence. Apoptotic cells were detected by staining of cleaved caspase 3, nuclei were stained with DRAQ5.

Journal: Oncogene

Article Title: Dissection of transcriptional and non-transcriptional p53 activities in the response to genotoxic stress.

doi: 10.1038/sj.onc.1209126

Figure Lengend Snippet: Figure 4 High p53 amounts provide an apoptotic signal. (a) Western blot: 18 h after transfection with GFP or HA-wtp53, NIH3T3 cells were mock () or 20 Gy gamma ( þ ) irradiated and harvested 26 h after irradiation. Lysates were probed for cleaved caspase 3, p53 and p21. Equal protein loading was confirmed by analysis of tubulin expression. (b) Immunostaining: 18 h after transfection with HA-wtp53 or -Gal-GFP, NIH3T3 cells were 20 Gy gamma-irradiated and fixed 26 h after irradiation. Transfected cells were identified with an HA-specific antibody or by GFP-fluorescence. Apoptotic cells were detected by staining of cleaved caspase 3, nuclei were stained with DRAQ5.

Article Snippet: Antibodies were obtained from Upstate: Bax (06- 499); Biomol: Cytochrome c (SA-226); Cell Signaling: Cleaved caspase 3 (9661) and Phospho-p53-Ser15 (9284); BD Pharmingen: p21 (556430); Oncogene: PIG3 (PC268) and Tubulin (CP06); Imgenex: Puma (IMG-459); Santa Cruz: 14-3-3s (sc7681).

Techniques: Western Blot, Transfection, Irradiation, Expressing, Immunostaining, Staining

Figure 5 Cytosolic p53 and Bax-activation in irradiated NIH3T3 cells. (a) Western blot: lysates from NIH3T3 cells were probed for cleaved caspase 3 at the indicated time points after 100 J/m2 UV-irradiation. Equal protein loading was confirmed by tubulin expression. (b) Western blot: NIH3T3 cells were irradiated as indicated and nuclear and cytosolic fractions were prepared 8 h after irradiation. Lysate (25 mg) were probed for p53. Purity of the fractions was confirmed by monitoring SAF-A and cytochrome c. (c–e) Confocal images: mitochondria and Bax were stained in untreated and irradiated NIH3T3 cells at different time points after irradiation. Representative cells are depicted. Bars are 5 mm. (c, d) Cells were fixed at different time points after 100 J/m2 UV- irradiation, either in an apoptotic (26 h) or pre-apoptotic state (8 and 12 h). (e) Gamma-irradiated cells (20 Gy) were fixed at the time of maximal Bax expression (26 h).

Journal: Oncogene

Article Title: Dissection of transcriptional and non-transcriptional p53 activities in the response to genotoxic stress.

doi: 10.1038/sj.onc.1209126

Figure Lengend Snippet: Figure 5 Cytosolic p53 and Bax-activation in irradiated NIH3T3 cells. (a) Western blot: lysates from NIH3T3 cells were probed for cleaved caspase 3 at the indicated time points after 100 J/m2 UV-irradiation. Equal protein loading was confirmed by tubulin expression. (b) Western blot: NIH3T3 cells were irradiated as indicated and nuclear and cytosolic fractions were prepared 8 h after irradiation. Lysate (25 mg) were probed for p53. Purity of the fractions was confirmed by monitoring SAF-A and cytochrome c. (c–e) Confocal images: mitochondria and Bax were stained in untreated and irradiated NIH3T3 cells at different time points after irradiation. Representative cells are depicted. Bars are 5 mm. (c, d) Cells were fixed at different time points after 100 J/m2 UV- irradiation, either in an apoptotic (26 h) or pre-apoptotic state (8 and 12 h). (e) Gamma-irradiated cells (20 Gy) were fixed at the time of maximal Bax expression (26 h).

Article Snippet: Antibodies were obtained from Upstate: Bax (06- 499); Biomol: Cytochrome c (SA-226); Cell Signaling: Cleaved caspase 3 (9661) and Phospho-p53-Ser15 (9284); BD Pharmingen: p21 (556430); Oncogene: PIG3 (PC268) and Tubulin (CP06); Imgenex: Puma (IMG-459); Santa Cruz: 14-3-3s (sc7681).

Techniques: Activation Assay, Irradiation, Western Blot, Expressing, Staining

Figure 7 3T3tx mutp53 triggers Bax-activation. (a) Western blot: The presence of cleaved caspase 3 was analysed in lysates from Balb/ c3T3tx cells at the indicated time points after 100 J/m2 UV-irradiation. (b) FACS analysis: DNA content was monitored in propidium iodide stained Balb/c3T3tx cells at the indicated time points post 100 J/m2 UV-irradiation. (c) Western blot: lysates from Balb/c3T3tx cells were probed for p53, serine 18 phosphorylated p53, and p53 targets Mdm2 (antibody 4B2) and Bax at the indicated time points after 100 J/m2 UV-irradiation. (d) Confocal images: mitochondria and Bax were stained in Balb/c3T3tx cells before or 6 h after 100 J/m2 UV-irradiation. Representative cells are depicted. Bars are 5 mm. (e) Confocal images: mitochondria, Bax and p53 were stained in Balb/ c3T3tx cells that were transiently transfected with p53-specific siRNA. A cell with downregulated mutp53 is depicted in higher magnification. Bars are 10 mm.

Journal: Oncogene

Article Title: Dissection of transcriptional and non-transcriptional p53 activities in the response to genotoxic stress.

doi: 10.1038/sj.onc.1209126

Figure Lengend Snippet: Figure 7 3T3tx mutp53 triggers Bax-activation. (a) Western blot: The presence of cleaved caspase 3 was analysed in lysates from Balb/ c3T3tx cells at the indicated time points after 100 J/m2 UV-irradiation. (b) FACS analysis: DNA content was monitored in propidium iodide stained Balb/c3T3tx cells at the indicated time points post 100 J/m2 UV-irradiation. (c) Western blot: lysates from Balb/c3T3tx cells were probed for p53, serine 18 phosphorylated p53, and p53 targets Mdm2 (antibody 4B2) and Bax at the indicated time points after 100 J/m2 UV-irradiation. (d) Confocal images: mitochondria and Bax were stained in Balb/c3T3tx cells before or 6 h after 100 J/m2 UV-irradiation. Representative cells are depicted. Bars are 5 mm. (e) Confocal images: mitochondria, Bax and p53 were stained in Balb/ c3T3tx cells that were transiently transfected with p53-specific siRNA. A cell with downregulated mutp53 is depicted in higher magnification. Bars are 10 mm.

Article Snippet: Antibodies were obtained from Upstate: Bax (06- 499); Biomol: Cytochrome c (SA-226); Cell Signaling: Cleaved caspase 3 (9661) and Phospho-p53-Ser15 (9284); BD Pharmingen: p21 (556430); Oncogene: PIG3 (PC268) and Tubulin (CP06); Imgenex: Puma (IMG-459); Santa Cruz: 14-3-3s (sc7681).

Techniques: Activation Assay, Western Blot, Irradiation, Staining, Transfection